FLUOROCHROMATIC EXAMINATION WITH MICROORGANISMS IN LIQUID MEDIA
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
About 15 fluorochromates are suitable for inducing satisfactory fluorescence in bacteria and other microorganisms in a liquid medium, in order to improve the conditions for automatic counting in continuous-flow chambers. This applies to living or dead bacteria. The most efficient terminal concentration for the dyes is 1 10,000. Fluorochromate solutions should be alkaline pH 9 but isotonia is not necessary. Under the selected specific experimental conditions, dead or living bacteria cannot be distinguished from each other on the basis of fluorescent reaction. Differentiation of individual types of micro organisms on the basis of fluorescent reaction is not possible in the present experimental arrangement. The disturbance through inherent fluorescence of the dye solutions can be reduced by elution and, to an even greater extent, by the addition of fluorescence-suppressants, e.g. aqueous 1- potassium iodide solutions.
- Medical Facilities, Equipment and Supplies