ISOLATION OF THE POLYHEDER VIRUS AND THE NATURE OF POLYHEDERS
JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA
Pagination or Media Count:
By treatment with dilute alkali and centrifuging, the substance of the polyheders found in moth larvae infected with the polyheder disease could be separated into a water-insoluble protein and a water-soluble nucleoprotein. The two moieties could be differentiated chemically on the basis of their phosphorus content, which was low in the case of the protein and high in the case of the nucleoprotein. It is concluded on the basis of experiments on insects that the nucleoprotein rather than the protein, which had been mistaken for the virus in earlier work, must be regarded as the causative factor of the virus infection known as polyheder disease. The nucleoprotein is likened to the smallpox soluble antigen, which can be separated by similar methods from the smallpox virus.