RADIOCHEMICAL INVESTIGATION OF THE BEHAVIOR OF INORGANIC IONS UNDER SEPHADEX-GEL FILTRATION. APPLICATION TO THE MICROCHEMICAL DETERMINATION OF PBI (PROTEIN-BOUND IODINE) IN BLOOD SERUM
ARMY BIOLOGICAL LABS FREDERICK MD
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In comparison with dialysis and the methods of protein precipitation, gel filtration offers considerable advantages in the removal of salts from protein solutions. The behavior of inorganic ions milligram to submicrogram amounts was studied on Sephadex micro-columns with the aid of radioactive isotopes. Inorganic ions were found to behave deversely depending on the concentration chosen and the swelling and elution liquid. In 0.9 sodium-chloride solution, sub microgram amounts of cations and anions also enter the gel phase they can be separated quantitatively and reproducibly from the protein which remains excluded from the gel phase. On the basis of the experimental data furnished, the gel filtration with Sephadex G-25 can be used for the separation of protein-bound serum iodine PBI from inorganic iodine. In contrast to the previous methods of separation, it is likewise useful for blood serums with extremely augmented iodide content. The saving in time and work is a further advantage.
- Radiation and Nuclear Chemistry