THERMAL STRESS ON CELLULAR STRUCTURE AND FUNCTION
PITTSBURGH UNIV PA
Pagination or Media Count:
Mouse kidney cells were grown on coverslips in a specially designed perfusion chamber which permitted continuous change of medium, microscopic examination and photography, and precise temperature measurement and control. Cells were exposed to normal body temperature for a time and then to an elevated temperature for a period of one to ten minutes. The temperature then was returned to normal. Observation of the cells revealed a standard pattern of response. Cells appear generally normal up to 42 C. Studies were made at 47, 50, 53, and 56 C. The time to reach any stage of damage is a factor of about 4. 5 for each 3 C increase in heat-shock. The duration of exposure to elevated temperature was found not to be as important as the maximum temperature reached. A series of curves showing the distribution of cells in various stages of cell damage with temperature is presented.
- Stress Physiology