Accession Number:

AD0292922

Title:

A FORCE-AREA STUDY OF MIXED FILMS OF CATALASE AND OVOMUCOID

Descriptive Note:

Corporate Author:

NAVAL RESEARCH LAB WASHINGTON D C

Personal Author(s):

Report Date:

1962-12-05

Pagination or Media Count:

9.0

Abstract:

An investigation of the surface chemical behavior of the enzyme-inhibitor complex between ovomucoid and catalase was undertaken. Monomolecular films of this complex were deposited on substrate solutions of ammonium sulfate at pH 4.0 and pH 7.0. Spreading solutions of several molecular ratios were employed. Force-area F-A curves for these films showed two inflections corresponding to different orientations of the film molecules and were similar in general shape to those obtained from films of the separate constituents. The force-area data were analyzed according to the method of Payens to determine the relation between the apparent molecular area of the complex and that which would be expected assuming no molecular interaction occurred between the two film components. This analysis indicated no evidence that molecular interaction was accompanied by an area change. Since such an interaction is known to take place, it is concluded that either the area occupied by the complex in the film is the sum of the areas of the separate components, or that the complex is so weakly bound that the spreading forces are sufficient to rupture the intermolecular bonds. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE