Accession Number:

AD0292679

Title:

THE STRUCTURE OF THE PHYCOBILINS

Descriptive Note:

Corporate Author:

UNIVERSITY COLL GALWAY (IRELAND)

Personal Author(s):

Report Date:

1962-09-29

Pagination or Media Count:

1.0

Abstract:

The literature concerned with the chemistry of algal biliproteins is surveyed. Amino acid analyses of carefully purified samples of Rand C-phycoerythrin, and of C-phycocyanin were performed. All three are characterised by a high percentage of dibasic and of hydrophobic amino acids. R-, B- and C-phycoerythrins were found to contain methionine as their N-terminal amino acid residue. A proteolytic enzyme preparation was obtained from the blue-green alga Phormidium persicinum. The phycoerythrobilin prosthetic group of R-phycoerythrin was purified and some of its physical and chemical properties determined. The urobilin obtained on isomerization of phycoerythrobilin in concentrated hydrochloric acid was studied, and a number of useful derivatives prepared. It was shown that 12N HCl is required to liberate the native prosthetic group of C-phycocyanin. Two other closely related pigments were isolated from this biliprotein and their physical and chemical properties studied. R-phycocyanin was shown to be a homogeneous chromoprotein containing subunits characteristic of phycoerythrin and of C-phycocyanin. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE