CHARACTERISTICS OF THE POLYSACCHARIDE-CONTAINING FRACTION FROM PASTEURELLA PESTIS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The preparation isolated by the White method from Past. pestis does not represent a pure polysaccharide. Non-purified fractions contained a considerable amount of protein admixtures, from which it could be separated via repeated, fractionated precipitation with alcohol, processing with chloroform as per Sevag, prolonged hydrolysis with 0.1 N acetic acid, or protein precipitation with trichloracetic acid. The preparation purified of protein admixtures represents a stable polysaccharide-polypeptide complex possessing serological activity and the property of inducing a positive allergic reaction in guinea pigs which had recovered from plague or had been immunized against this infection. Removal of the polypeptide portion of the complex by means of trypsin-processing led to a marked reduction of the allergic effect of the preparation.