EFFECTS OF SUGARS ON THE SURVIVAL OF SERRATIA MARCESCENS AFTER FREEZE DRYING OR AEROSOLIZATION AT UNFAVORABLE HUMIDITY
Technical manuscript no. 6
ARMY BIOLOGICAL LABS FREDERICK MD
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Suspensions of Serratia marcescens were subjected to freeze drying or to aerosolization at unfavorable humidity levels. The survival of the cells during these treatments was markedly improved in the presence of common sugars, but no one sugar stabilized the cells against both stresses. The protective effects of the sugars were correlated with their penetrability into cells minimally penetrable MP sugars stabilized cells against aerosolization and freely penetrable FP sugars stabilized cells during freeze drying. These results were attributed to the modifications of intracellular water content induced by the presence of the sugars in the cell suspensions.
- Chemical, Biological and Radiological Warfare