Accession Number:

AD0271443

Title:

THE MEASUREMENT OF DEXTROSE IN STANDARD SOLUTIONS WITH DREYWOOD'S ANTHRONE REAGENT AND THE KLETT-SUMMERSON PHOTOELECTRIC COLORIMETER (A STATISTICAL EVALUATION AND A SIMPLE MICROPROCEDURE)

Descriptive Note:

Corporate Author:

NAVAL MEDICAL RESEARCH LAB NEW LONDON CT

Report Date:

1958-04-10

Pagination or Media Count:

16.0

Abstract:

An experiment was performed using a macro-procedure for the anthrone-hexose reaction with standard solutions of dextrose and a Klett-Summerson photoelectric colorimeter in order to reveal significant sources of error and variability and to assess the upper limits of accuracy of this reaction with reference to its suitability for the clinical laboratory and other places where precision may be required. Sensitivity and accuracy were found sufficient to permit the introduction of additional errors arising from the use of biological fluids without necessarily removing the anthrone-method from a position of advantage in competition with other procedures. significant sources of error and variability and to assess the upper limits of accuracy of this reaction with reference to its suitability for the clinical laboratory and other places where precision may be required. Sensitivity and accuracy were found sufficient to permit the introduction of additional errors arising from the use of biological fluids without necessarily removing the anthrone-method from a position of advantage in competition with other procedures. Slow fading of the anthrone-carbohydrate complex occurred after 8 hours at 24 deg C but this fading was undetectable if specimens were read as soon as they cooled. When special reaction tubes designed to conserve heat were used in a warm room, the anthrone-hexose reaction obeyed the Bouguer-Beer law for dextrose in quantities of 3.75 to 20 gamma in 0.75 ml. of original solution. Two supplementary methods of controlling temperature to obtain satisfactory color development are considered, and the effects of their improper use are pointed out. A simple procedure is proposed for using Dreywoods anthrone reagent to measure sugar in 20 cu. mm. of blood collected in a Sahli pipette.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE