Accession Number:

AD0266165

Title:

MICROBIOLOGIC STUDIES WITH OZONE MUTAGENESIS OF OZONE FOR ESCHERICHIA COLI

Descriptive Note:

Corporate Author:

SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s):

Report Date:

1961-06-01

Pagination or Media Count:

1.0

Abstract:

This report is one of a series of tudies d signed to provide an un erstanding of the biologic activity of ozone at the cellular level. The possible role of ozone as a biologic mutagenic agent was undertaken. Ozone was produced by a Welsbach water-cooled corona discharge ozonator using dry, clean oxygen. Analyses for ozone in aqueous solutions were carried out spectrophotometrically. Cells of Escherichia coli were exposed to aliquots of ozonated-wa er stock solutions. Bacterial survivors and mutants were determined by recognized plating procedures. T HE MUTAGENESIS OF OZONE IN AQUEOUS SOLUTIONS ON E. coli was studied in two mutational systems. Mutation frequencies in the streptomycin-dependence method appear o increase with increasing concentrations of ozone and decreasing survival. In the phage-resistance me hod, the frequency of zero-point mutants showed a significant increase after exposure of the cells to ozone. The importance of free radicals in biologic processes is discussed. It is suggested that a common pathway exists in the formation of these active radicals following irradiation, ozonization, or oxygen poisoning of biologic specimens. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE