Accession Number:

AD0266104

Title:

STERILIZATION OF PLASMA PROTEINS

Descriptive Note:

Corporate Author:

PROTEIN FOUNDATION LABS JAMAICA PLAIN MASS

Personal Author(s):

Report Date:

1961-11-03

Pagination or Media Count:

1.0

Abstract:

Three tech ique w re studied for re ucing the possibility of transmission of viruses in the clinical use of plasma proteins. Heat Sterilization Optimal conditions were found for heating solutions of transferrin and of gamma globulin for 10 hours at 60 C., a procedure that has been shown to destroy the virus of serum hepatitis. Transferrin app rs o be un l ere by the heating procedure. Gamma globulin solutions retain antibody titers but are more viscous following heating. Millipore Filtration Of six proteins studied, solutions of albumin, gamma globulin, fibrinogen and properdin, but not of beta-lipoprotein or Factor V AcG will pass through Millipore V F filter sheets with purported pore size of 10 millimicron, a dimension smaller than that of any known virus. Acceptance of the technique will depend upon demonstration of the failure of hepatitis virus to pass such filters. Ethylene oxide Gas Using C14 labelled ethylene oxide conditions have been determined permitting maximum interaction of the gas with lyophilized nucleic acids and minimum interaction with lyophilized plasma proteins. The technique has been shown to inactivate tobacco mosaic virus. Experiments with inactivation of coxsackie virus in plasma have been equivocal. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE