HAPTENIC PROPERTIES OF PARALYTIC SHELLFISH POISON
Quarterly progress rept. no. 3, 1 Jan-31 Mar 1961
ROBERT A TAFT SANITARY ENGINEERING CENTER CINCINNATI OH
Pagination or Media Count:
Through additional modification of the procedures for coupling diazo- PSP to proteins, a product has been synthesized of sufficiently low toxicity to be acceptable for use as an antigen and containing at least 10 times the amount of PSP as previous preparations. Immunological studies of PSP-bovine gamma II globulin, PSP-ovalbumin and their respective controls provided conflicting evidence regarding the haptenic properties of the PSP portion of the conjugates yet to be reconciled. Evidence has been presented to indicate PSP enters into a reversible equilibrium reaction with nitrous acid and that the diazotization does not appear to drastically alter the PSP molecule. In search of alternate methods for chemical assay procedures, a color reaction between PSP and sodium 1,2,-napthoquinone-4-sulfonate at pH 9.3 has been investigated in terms of specificity and sensitivity.