HAPTENIC PROPERTIES OF PARALYTIC SHELLFISH POISON
Quarterly progress rept. no. 2, 1 Oct-31 Dec 1960
ROBERT A TAFT SANITARY ENGINEERING CENTER CINCINNATI OH
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The purpose of this project is a to determine the feasibility of joining the toxin of Gonyaulax catenella with other molecules to produce one or more conjugates having immunogenic properties, b to develop a specific micro- assay method for paralytic shellfish poison based on immunological reactions, and c to lay the ground work for immunization of humans against the poison. Through modifications of procedures described previously, a technique has been developed for conjugating diazotized paralytic shellfish poison PSP to protein so that the final product is of sufficiently low toxicity to be suitable for use as an antigen. Immunological investigations of PSP-ovalbumin and PSP-bovine gamma II globulin reveal that both conjugates will elicit an antibody response in rabbits. Although it was not possible to demonstrate clearly that the PSP portion of the molecule had haptenic properties, evidence was developed suggesting this to be the case.
- Undersea and Antisubmarine Warfare