Accession Number:

AD0261404

Title:

ENZYMATIC STUDIES IN LIVER DISEASE. STUDIES OF THYMOL AND ZINC TURBIDITY TESTS

Descriptive Note:

Corporate Author:

PENNSYLVANIA UNIV PHILADELPHIA

Personal Author(s):

Report Date:

1961-05-31

Pagination or Media Count:

1.0

Abstract:

Following administration of c rbon tetrachloride to mice, lowered concentrations of dihydrodipho phopyridine nucleotides DPNH concentrations were observed in liver. Diphosphopyridine nucleotide DPN concentrations were unchanged. Ammonium concentrations were the same in injured livers as controls. However, ammonium formation was greater in livers of CCl4 treated mice, an effect attributed to decreased ammonium removal in part because of impaired glutamic dehydrogenase activity. Addition of DPNH and alpha ketoglutarate, substrates for glu amic dehydrogenase, prevented further accumulation of ammonium in br is of injured livers. DPNH alone was less effective. Patients in hepatic coma tended to have high DPN concentrations in erythrocytes. Therapeutic administration of nicotinic acid was a contributing cause. Tissue breakdown may have provided substrates for increased synthesis. A major source of error in blood and tissue ammonia measurements was identified, namely, decomposition of protein by alkali. A revised method was developed. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE