Accession Number:

AD0261233

Title:

FATTY ACID OXIDATION BY MITOCHONDRIA FROM COLD-FASTED RATS

Descriptive Note:

Corporate Author:

ARCTIC AEROMEDICAL LAB FORT WAINWRIGHT ALASKA

Personal Author(s):

Report Date:

1961-04-01

Pagination or Media Count:

1.0

Abstract:

Mitochondria from cold-fasted rats oxidized about as much acetate-1-C14 to C14O2 as did mitochondria from control rats when the data were expressed on the basis of dry weight of the mitochondria. This was a most surprising result because liver slices wet weight basis and liver homogenates protein content basis from coldfasted rats form far less C14O2 from acetate-1C14 than do similar preparations from control rats. The possible reasons for these differences in results with liver slices and liver homogenates on the one hand and liver mitochondria on the other are discussed. Mitochondria from coldfasted rats were also found to oxidize palmitate1-C14 to C14O2 as rapidly as the mitochondria from control rats, thereby confirming the results obtained with liver slices. Carbohydrate metabolism failed to promote palmitate oxidation by mitochondria from cold-fasted rats just the opposite result is obtained with liver slices. The use of mitochondria to study the regulation of multistep metabolic pathways is discussed. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE