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Research Quarterly Progress Report, 16 January-15 April 1961 carried out by the Sanitary Engineering Center, U.S. Public Health Service

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Field samples, C3 and D3, were compared with previous standards C2 and D2 for antigen content. The new standards contained essentially the same amount of antigenic activity. Several cellulose ion exchangers, and Dow ion exchange resins were evaluated for their selectivity in removing the antigenic components from field sample D2. Activated carbon was investigated on the basis that phenol will remove the activity of sample C2 from an aqueous solution and, therefore, it might possibly desorb the antigens from carbon. The feasibility of using ion exchange resin columns in series was investigated. The aim of this method was to increase the efficiency of the borate form of CG-400 resin in river water by first removing interfering substances. Although the yield was not great, this method would offer a means of detection. With an unlimited amount of sample, such as would be encountered in a river, the success of this method would depend upon the performance of the mixed bed exchanger. Some time was devoted to the recovery of antigenic materials from soil. This line of investigation seems profitable because of the natural ion exchange properties of most soils and also the advantage of transporting soil samples. A sample of soil was received from an area known to have been exposed to B. suis and P. tularensis. Of the various elution procedures tried, soaking with distilled water at pH 2.5 was the most successful. Author

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  • Biochemistry
  • Physical Chemistry

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