Accession Number:

AD0256394

Title:

THE INFLUENCE OF VARIOUS METABOLITES ON THE GROWTH OF COXIELLA BURNETTI IN MONOLAYER CULTURES OF CHICK EMBRYO ENTODERMAL CELLS

Descriptive Note:

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s):

Report Date:

1960-11-21

Pagination or Media Count:

1.0

Abstract:

The factors influencing the growth of Coxiella burnetti in chick embryo entodermal cell monolayers were investigated. The methods of preparation and infection of the cultures and determination of extent of rickettsial growth were similar, but somewhat simpler than those reported by Weiss and Pietryk 1956. The addition of Morgans mixture 199 or the constituents of Eagles medium to a basal medium composed of untreated chicken serum and Hanks BBS significantly inhibited rickettsial growth. The amino acids and glutamine of Eagles medium proved to be the inhibitory factors. Rickettsial growth was also greatly depressed in the absence of serum or in the presence of dialyzed or chloroform-treated serum. The addition of individual metabolites produced variable results. Pyruvate yielded rather consistent results. Enhancement or inhibition was obtained within a very small range of concentrations, 2.5 to 0.75 mM enhancing and 10 mM usually inhibiting. Glucose did not affect the growth of the rickettsiae or influence the enhancing effect of pyruvate. In one experiment DPN was inhibitory in concentrations 0.1 and 0.05 mM but enhancing at 0.025 mM. Although somewhat similar results were obtained with ATP and CoA this effect has not been reproducible. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE