STUDY OF CHEMICAL METHODS FOR QUANTITATIVE MEASUREMENTS OF CATECHOL AMINES
MASSACHUSETTS GENERAL HOSPITAL BOSTON BOSTON United States
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A method for the estimation of adrenaline and noradrenaline in urine is presented. This method employs an ion exchange resin, Amberlite XE-64, for purification. The simultaneous estimation of the two hormones is accomplished by a multiple filter technique of fluorometry. The selection of appropriate sets of filters is based on a careful study of the fluorescence spectra of the compound concerned. The method has been subjected to careful scrutiny as to factors affecting reproducibility and precision of the measurements. Evidence is presented that a high degree of specificity has been attained.