FLOW BIREFRINGENCE STUDIES IN SOLUTIONS OF MACROMOLECULES
Annual rept. 1 Jan-31 Dec 1952
CORNELL UNIV ITHACA NY
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Studies were made to determine the thermodynamic parameters characterizing the equilibria involved in the thrombin-induced fibrinogen-fibrin conversation. Peptide bonds, broken in the liberation of the polypeptide from the fibrinogen molecule, appeared much stronger than the usual bonds in simple peptides. Flow birefringence studies indicated the polymer distribution of activated fibrinogen below pH 6 appeared of low molecular weight as compared to the high molecular weight of polymers between pH 6 and 10. Flow birefringence and viscosity results suggested that the fibrinogen molecule may be split portions. Hydrodynamic and IR measurements identified the structural changes produced during the methylation of bovine serum albumin as esterifications of carboxyl groups. A quantitative correlation was obtained between the optical densities in the IR absorption spectra and the number of methoxyl groups.