Accession Number:

AD0002144

Title:

THE LABORATORY PRODUCTION OF GONYAULAX CATANELLA POISON

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Report Date:

1952-11-06

Pagination or Media Count:

50.0

Abstract:

Methods were developed for estimating Gonyaulax catanella growt h by direct microscopic count or by the analytical determination of cellular carbonhydrate. Use of a large inoculum, illumination with fluorescent light, and continuous incubation at 17 deg C for 7-14 days gave maximal yields of 50,000- 80,000 cellsml in sea water and in artificial sea-water media. The addition of culture filtrates, extracts of contaminant bacteria, or organic nutrients to sea-water media did not alter growth. Addition of polymyxin B, penicillin, streptothricin, or streptomycin improved the growth. Contact with rubber products or galvanized sheet metal completely inhibited growth. Stainless steel and polyethylene inhibition were overcome with ethylene-diaminetetraacetate EDTA neither Al nor saran caused inhibition. Growth in the sea salt medium was improved by the addition of 0.01 EDTA and 5-15 of sea water. The contaminants in the stock G, catanella culture were not eliminated by treatment with antibiotics, washing processes, or UV irradiation. The best poison yield, 1.3 mouse unitsml, was obtained in 3.5 l of sea-water culture containing 17 Gamma of polymyxin Bml after 7 days of incubation in a rotating fermenter with continuous fluorescent illumination.

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE