Accession Number : ADP005147


Title :   Comparison of Analysis and Quantification of Cell Death in vivo and in vitro


Corporate Author : NORTH CAROLINA UNIV AT CHAPEL HILL DEPTOF PATHOLOGY


Personal Author(s) : Grisham, Joe W ; Smith, Gary J ; Beliles, Karen E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/p005147.pdf


Report Date : May 1985


Pagination or Media Count : 13


Abstract : Cell death and related cytotoxic responses are frequent cellular reactions to toxic chemicals. Cell culture systems allow more accurate quantitation of cell death and related reactions in response to chemicals than do animals. However, mammalian cell culture systems do not yet provide a solution for many requirements of toxicity testing. Cell culture systems are unable to reflect distribution and organ-specific metabolic activation of protoxic chemicals that are typical of different animals species. Thus, cell culture systems do not replace the need for animals in toxicity studies, but when used in conjunction with limited studies in animals cell culture assays can improve the sensitivity of detection of chemical toxicity and provide more detailed understanding of cellular mechanisms of toxic responses.


Descriptors :   *TOXICITY , *DEATH , *CELLS(BIOLOGY) , TISSUE CULTURE CELLS , SYMPOSIA , IN VITRO ANALYSIS , ANIMALS , IN VIVO ANALYSIS , TOXICOLOGY , BIOASSAY , APOPTOSIS


Subject Categories : Stress Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE