Accession Number : ADB263763


Title :   Tissue Engineering Initiative


Descriptive Note : Annual rept. 1 Aug 1999-31 Jul 2000


Corporate Author : CLEVELAND CLINIC FOUNDATION OH


Personal Author(s) : Hascall, Vincent C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b263763.pdf


Report Date : Aug 2000


Pagination or Media Count : 375


Abstract : The Cleveland Clinic Foundation Tissue Engineering Initiative was proposed to initiate novel tissue engineering projects as a collaboration between connective tissue biochemists, bioengineers, prototype engineers and physician researchers from the Department of Biomedical Engineering. The primary focus of these projects was to test applications of gels consisting of purified hyaluronan, covalently crosslinked by divinyl sulfone (hylans). The gel formulations tested were obtained via collaboration with Biomatrix, Inc. During the first year of this project, considerable effort has been expended evaluating the biochemical makeup of these gels, as well as their biomechanical properties. Additionally, a set of experiments was designed to evaluate the ability of cells to attach to the gels and proliferate. It was found that untreated gels did not support good cell attachment. However, when surface coated with cell adhesion proteins (collagen I, extracellular matrix (ECM) gel, laminin, fibronectin), cells attach and proliferate actively. The mechanical property evaluations and cell attachment studies show properties necessary for the use of hylans as substrates for the development of tissue engineered cell-hylan composites. However, current hylan formulations have demonstrated some limitations to the proposed applications. Methods are currently under investigation to improve the properties of hylans to better suit the proposed tissue engineering applications.


Descriptors :   *TISSUES(BIOLOGY) , *CROSSLINKING(CHEMISTRY) , *EPIDERMIS , *BIOENGINEERING , *CONNECTIVE TISSUE , TEST AND EVALUATION , MECHANICAL PROPERTIES , BIOCHEMISTRY , PROTEINS , FORMULATIONS , TEST METHODS , GLYCOPROTEINS , PROTOTYPES , PHYSICIANS , ELECTRONIC COUNTERMEASURES , CELLS(BIOLOGY) , GELS , BIOMEDICINE , VINYL RADICALS , BONES , SULFONES , MEDICAL ENGINEERING


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE