Accession Number : ADB228766


Title :   Molecular Mechanisms of Metastasis Suppression in Human Breast Cancer


Descriptive Note : Annual rept. 1 Jul 1996-30 Jun 1997


Corporate Author : PENNSYLVANIA STATE UNIV UNIVERSITY PARK


Personal Author(s) : Welch, Danny R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b228766.pdf


Report Date : Jul 1997


Pagination or Media Count : 101


Abstract : The major cause of cancer deaths can be attributed to metastasis. Our goal is to identify metastasis-controlling genes for human breast cancer. This research is based upon our finding that microcell-mediated transfer of chromosome 11 into MDA-MB-43 5 results in nearly complete suppression of metastasis without suppressing tumorigenicity. The key findings during this reporting period were: (1) demonstration that KiSS-i, a melanoma metastasis- suppressor gene, caused significant suppression of metastatic potential of MDA-MB-43 5 human breast carcinoma cells; (2) demonstration that Kai-1 niRNA expression levels correlated with metastatic potential of a panel of human breast carcinoma cell lines; and (3) showing that transfection of Kai-1 resulted in partial suppression of metastasis. Preliminary results include: (1) Using differential display, we also identified several differentially expressed genes in neo 11/435 hybrids compared to controls. Initial findings were complicated by overabundance of retroviral sequences (presumably resulting from method of 11 donor derivation). However, some cDNAs fragments were apparently novel. Further characterization is underway. (2) To prepare for transfer into NIDA- MB-435, we identified and characterized a panel of chromosome 11 with deleted regions. Some hybrids have been made and are being characterized.


Descriptors :   *MOLECULAR PROPERTIES , *SUPPRESSION , *METASTASIS , *BREAST CANCER , HUMANS , DISPLAY SYSTEMS , GENES , CANCER , SUPPRESSORS


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE