Accession Number : ADB226038


Title :   Biodegradable Bioadherent Microcapsules for Orally Administered Sustained Release Vaccines


Descriptive Note : Annual rept. 29 Apr 1996-28 Apr 1997


Corporate Author : LYNNTECH INC COLLEGE STATION TX


Personal Author(s) : Giletto, Anthony


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b226038.pdf


Report Date : May 1997


Pagination or Media Count : 34


Abstract : This research focuses on the development of an orally administered microencapsulated vaccine against botulinum neurotoxin type A using the non-toxic C-fragment of the neurotoxin (Bot-Ac). The research focuses on the production through recombinant techniques and the incorporation of a known bioadhesive, vitelline protein B (vpb), into the microsphere preparations to enhance uptake of the microspheres by the M cells located in the Peyer's patches. The results demonstrate that vpB can be produced at 170-350 mg/L in a Pichia pastoris expression system. iso, microspheres that contain 10% vpB generate a higher serum IgG level than microspheres that do not contain - B when administered either orally or subcutaneously. Whether vpB is acting as a true bioadhesive is still under study through the use of Peyer's patch uptake experiments. For comparison it was also determined that when 2.0 g of 10 mm microspheres containing 89.9% BSA /10.0% vpB /0.1% Bot-Ac were administered subcutaneously, detectable serum IgG level was generated at 17 weeks post-immunization.


Descriptors :   *BLOOD SERUM , *TOXINS AND ANTITOXINS , *VACCINES , *VITELLINE GLANDS , PREPARATION , CELLS , PROTEINS , RELEASE , ENCAPSULATION , CNS DEPRESSANTS , MICROSPHERES


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE