Accession Number : ADB173090


Title :   Electron Microscopy to Correlate Cell Structure and Biochemical Activity


Descriptive Note : Final rept. 15 Nov 1989-14 Nov 1992


Corporate Author : CASE WESTERN RESERVE UNIV CLEVELAND OH INST OF PATHOLOGY


Personal Author(s) : Aikawa, Masamichi


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b173090.pdf


Report Date : 10 Mar 1993


Pagination or Media Count : 33


Abstract : We performed immuno-electron microscopy to study intracellular localization and fate of liposome-encapsulated malarial antigen after phagocytosis by macrophages. Liposome-encapsulate protein that is phagocytosed by macrophages can enter an intracellular compartment in which at least some of the antigenic epitopes are not degraded by lysosomal enzymes. We also localized P. falciparum antigen having molecular weight 175 kDa (EBA) within the parasite by immunoelectron microscopy. EBA is specifically localized in micronemes of P. falciparum merozoites. The CS protein, SSP2, the Duffy receptor and now EBA-175 have been identified in the micronemes, suggesting that these organelles play a critical role in the storage and release of parasite proteins that act as receptors for invasion into host cells. P. coatneyl produces knobs on the membrane of PRBC and these PRBC appear to sequester in the vasculature of infected rhesus monkeys. We studied the pathology of CNS of rhesus monkeys infected with P. coatneyl and found that cytoadherence of PRBC to endothelial cells is a consistent feature of infections with this primate parasite. Cerebral microvessels with sequestered PRBC were shown by immunohistochemistry to possess CD36, TSP and ICAM-1. Our study indicates for the first time, that rhesus monkeys infected with P. coatneyl can be used as a primate model to study human cerebral malaria. Malaria, Immunoelectron-microscopy, Chemotherapy, DFO, Cerebral malaria, RA 1.


Descriptors :   *ANTIMALARIALS , *ELECTRON MICROSCOPY , *MALARIA , ANTIGENS , BIOCHEMISTRY , CELLS(BIOLOGY) , CEREBRUM , CHELATION , CHEMOTHERAPY , COMPARTMENTS , CONTROL KNOBS , DEGRADATION , ENDOTHELIUM , ENZYMES , HUMANS , INHIBITION , LIPOSOMES , MACROPHAGES , MEMBRANES(BIOLOGY) , MEROZOITES , MODELS , MOLECULAR WEIGHT , PARASITES , PATHOLOGY , PHAGOCYTES , PLASMODIUM FALCIPARUM , PRIMATES , PROTEINS , RETICULOENDOTHELIAL SYSTEM , RHESUS MONKEYS


Subject Categories : Medicine and Medical Research
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE