Accession Number : ADB161569


Title :   Production and Characterization of an Avian Ricin Antitoxin


Descriptive Note : Final rept. 15 Jul 1991-15 Jan 1992 on Phase 1


Corporate Author : OPHIDIAN PHARMACEUTICALS INC MADISON WI


Personal Author(s) : Stafford, Douglas C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b161569.pdf


Report Date : 15 Jan 1992


Pagination or Media Count : 24


Abstract : This study was designed to demonstrate the feasibility of producing a ricin antitoxin consisting of highly purified specific avian antibodies. Antitoxins consisting of avian antibodies have significant advantages when compared to conventional mammalian (e.g., horse) antibodies; (a) avian antibodies do not fix human complement, eliminating the associated risk of inflammatory reactions, and, (b) avian antibodies can be manufactured more economically. This study also utilized affinity chromatography to increase antibody purity, increasing effectiveness and reducing the risk of serum sickness in the patient. Ricin toxoid produced by formalin treatment was injected into laying hens followed by collection of eggs bearing hyperimmune antibody. Antibody was extracted from the yolks and assayed by enzyme immunoassay. When suitable antibody was measured in the eggs, extracted immunoglobulin was further purified by affinity chromatography using immobilized ricin.


Descriptors :   *BIRDS , *RICIN , *TOXINS AND ANTITOXINS , RISK , PRODUCTION , ENZYMES , PURITY , PURIFICATION , EXTRACTION , ANTIBODIES , CHICKENS , EGGS , IMMUNOASSAY , INFLAMMATION , CHROMATOGRAPHY , IMMUNOGLOBULINS


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE