Accession Number : ADB101431


Title :   Production of a Suitable Antibody and an Enzyme Immunoassay Kit for the Field Detection of T-2 Toxin


Descriptive Note : Annual summary rept. 1 Dec 1982-30 Nov 1983


Corporate Author : VESTAR RESEARCH INC PASADENA CA


Personal Author(s) : Callahan, Richard A ; Richer, LeRoy L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/b101431.pdf


Report Date : 25 Oct 1983


Pagination or Media Count : 22


Abstract : Phospholipid vesicles, liposomes, were used as an immune enhancement (adjuvant) medium to produce antibodies specific for T-2 toxin. Vesicles were prepared such that neat T-2 toxin and a solution of T-2 succinyl bovine serum albumin were each either encapsulated or adsorbed on vesicles. Permutations of these formulations were used to immunize mice by repeated subcutaneous injections. An enzyme-linked immunosorbent assay was developed and used to assay serum from immunized mice for T-2 specific antibody. The assay allows for direct measurement of T-2 antibody, with the capability for competitive inhibition studies using T-2 standard solutions. Two objectives were specified for this study. They were to produce antibodies specific for T-2 toxin, and to demonstrate an immune enhancement, with respect ot T-2 antibody production, from the vesicle-based antigen delivery method. The vesicle delivered antigen does produce T-2 specific antibodies to T-2 toxin. Potential benefit of using the vesical base immunization method of increasing the rate of antibody production or increasing antibody titers is still under study. Keywords: Vesicle microencapsulation; Enzyme-Linked Immunosorbent Assay (ELISA); and Field detection.


Descriptors :   *ANTIBODIES , *IMMUNITY , *IMMUNOASSAY , *PHYTOTOXINS , ASSAYING , BLOOD SERUM , CYTOPLASM , DETECTION , ENZYMES , FIELD CONDITIONS , IMMUNIZATION , INHIBITION , KITS , MEASUREMENT , MICE , OPTIMIZATION , PERMUTATIONS , PRODUCTION , RATES , TITRATION


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE