Accession Number : ADA635002


Title :   Carbon Tetrachloride Increases Intracellular Calcium in Rat Liver and Hepatocyte Cultures


Descriptive Note : Doctoral thesis


Corporate Author : UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD


Personal Author(s) : Long, Rochelle M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a635002.pdf


Report Date : 12 May 1986


Pagination or Media Count : 163


Abstract : Sequestration of ionized calcium (Ca++) by rat liver endoplasmic reticulum (ER) is inhibited following in vivo administration of the hepatotoxin carbon tetrachloride (CC14 ). Consequently, hepatic cytosolic Ca++ may rise to supraphysiologic levels, perhaps leading to excessive stimulation of Ca++-sensitive processes occurring within liver cells. The hypothesis examined was whether cytoplasmic Ca++ concentration. are elevated in rat liver and hepatocyte cultures exposed to CCl4 Ca++ levels were determined indirectly via the Ca++_dependent conversion of glycogen phosphorylase to the form, and directly with the fluorescent Ca++-indicator compound quin2. Other biochemical alterations produced by CCl4 were also monitored in order to relate Ca++ increases to the development of hepatotoxicity. Within half an hour after administration of CCl4 to rats (1.5 ml/kg body weight), liver phosphorylase levels increased (136% of control), liver glycogen concentrations decreased (66% of control), and ER Ca++ pump activity was inhibited (35% of control). These effects persisted through 24 hours. Hepatic glucose-6-phosphatase activity did not decrease until two hours (71% of control), and 5'-nucleotidase activity never changed. By eight hours, serum glutamic-pyruvic transaminase activity and total liver calcium levels were elevated (939% and 450% of control, respectively).


Descriptors :   *CALCIUM , *CARBON TETRACHLORIDE , *LIVER , ASSAYING , CELLS(BIOLOGY) , CONCENTRATION(CHEMISTRY) , CULTURES(BIOLOGY) , HOMEOSTASIS , RATS , TOXICITY


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Inorganic Chemistry
      Organic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE