Accession Number : ADA634068


Title :   Differential Expression of Inward and Outward Potassium Currents in the Macrophage-like Cell Line J774.1


Descriptive Note : Doctoral thesis


Corporate Author : UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD


Personal Author(s) : Sheehy, Paul A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a634068.pdf


Report Date : 02 Apr 1985


Pagination or Media Count : 151


Abstract : The electrophysiological properties of the J774.1 macrophage-like cell line were studied at 25 deg C. Both conventional intracellular microelectrode and whole-cell patch microelectrode techniques were used. Long-term adherent (24 hours) cultures investigated with intracellular electrodes exhibited a broad distribution of resting membrane potentials and membrane resistances (RMP approximately -50 +/- 3.0 mV, Rm approximately 117 +/- 13 Mohm, Mean +/- SEM, n approximately 55). Most cells with hyperpolarized RMPs exhibited inward rectification.


Descriptors :   *CELLS(BIOLOGY) , *ELECTROPHYSIOLOGY , *MACROPHAGES , *POTASSIUM , DISTRIBUTION , ELECTRODES , MEDICAL RESEARCH , MEMBRANES , RESISTANCE , THESES


Subject Categories : Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE