Accession Number : ADA630422


Title :   Hemodynamic Changes in Rat Leg Muscles during Tourniquet-induced Ischemia-reperfusion Injury Observed by Near-infrared Spectroscopy


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Kim, J G ; Lee, J ; Roe, J ; Tromberg, B J ; Brenner, M ; Walters, T J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a630422.pdf


Report Date : 01 Jul 2009


Pagination or Media Count : 16


Abstract : In this study, we hypothesized that non-invasive continuous wave near-infrared spectroscopy (CWNIRS) can determine the severity or reversibility of muscle damage due to ischemia/reperfusion (I/R), and the results will be highly correlated with those from physical examination and histological analysis. To test this hypothesis, we performed CWNIRS measurements on two groups of male Sprague-Dawley rats (approximately 400 g) that underwent 2 h ( n = 6) or 3 h ( n = 7) of pneumatic tourniquet application (TKA). Tissue oxyhemoglobin [HbO 2 ] and deoxyhemoglobin [Hb] concentration changes were monitored during the 2 h or 3 h of 250 mmHg TKA and for an additional 2 h post- TKA. Rats were euthanized 24 h post-TKA and examined for injury, edema and viability of muscles. Contralateral muscles served as controls for each animal. In both groups, [HbO 2 ] dropped immediately, then gradually decreased further after TKA and then recovered once the tourniquet was released. However, releasing after 2 h of TKA caused [HbO 2 ] to overshoot above the baseline during reperfusion while the 3 h group continued to have lower [HbO 2 ] than baseline. We found a significant correlation between the elapsed time from tourniquet release to the first recovery peak of [HbO 2 ] and the muscle weight ratio between tourniquet and contralateral limb muscles ( R = 0.86). Hemodynamic patterns from non-invasive CWNIRS demonstrated significant differences between 2 h and 3 h I/R. The results demonstrate that CWNIRS may be useful as a non-invasive prognostic tool for conditions involving vascular compromise such as extremity compartment syndrome.


Descriptors :   *ISCHEMIA , *WOUNDS AND INJURIES , BLOOD CIRCULATION , CARDIOVASCULAR SYSTEM , CLINICAL MEDICINE , CONTINUOUS WAVES , EDEMA , EXTREMITIES , HISTOLOGY , MEDICAL SUPPLIES , MUSCLES , NEAR INFRARED RADIATION , PATTERNS , PERFUSION , PHYSIOLOGICAL EFFECTS , RATS , RECOVERY , SIGNS AND SYMPTOMS , SPECTROSCOPY , TISSUES(BIOLOGY)


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Atomic and Molecular Physics and Spectroscopy


Distribution Statement : APPROVED FOR PUBLIC RELEASE