Accession Number : ADA629500


Title :   Repair of Traumatic Skeletal Muscle Injury with Bone-Marrow-Derived Mesenchymal Stem Cells Seeded on Extracellular Matrix


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Merritt, Edward K ; Cannon, Megan V ; Hammers, David W ; Le, Long N ; Gokhale, Rohit ; Sarathy, Apurva ; Song, Tae J ; Tierney, Matthew T ; Suggs, Laura J ; Walters, Thomas J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a629500.pdf


Report Date : 02 Jun 2010


Pagination or Media Count : 13


Abstract : Skeletal muscle injury resulting in tissue loss poses unique challenges for surgical repair. Despite the regenerative potential of skeletal muscle, if a significant amount of tissue is lost, skeletal myofibers will not grow to fill the injured area completely. Prior work in our lab has shown the potential to fill the void with an extracellular matrix (ECM) scaffold, resulting in restoration of morphology, but not functional recovery. To improve the functional outcome of the injured muscle, a muscle-derived ECM was implanted into a 1 X 1cm2 , full-thickness defect in the lateral gastrocnemius (LGAS) of Lewis rats. Seven days later, bone-marrow-derived mesenchymal stem cells (MSCs) were injected directly into the implanted ECM. Partial functional recovery occurred over the course of 42 days when the LGAS was repaired with an MSC-seeded ECM producing 85.4 + or - 3.6% of the contralateral LGAS. This was significantly higher than earlier recovery time points ( p less than 0.05). The specific tension returned to 94 + or - 9% of the contralateral limb. The implanted MSC-seeded ECM had more blood vessels and regenerating skeletal myofibers than the ECM without cells ( p less than 0.05). The data suggest that the repair of a skeletal muscle defect injury by the implantation of a muscle-derived ECM seeded with MSCs can improve functional recovery after 42 days.


Descriptors :   *MUSCULOSKELETAL SYSTEM , *REGENERATIVE MEDICINE , *REPAIR , *STEM CELLS , *TRAUMA , *WOUNDS AND INJURIES , BLOOD VESSELS , BONE MARROW , CELLS(BIOLOGY) , CONNECTIVE TISSUE , EMBRYOS , EXTREMITIES , IMPLANTATION , MORPHOLOGY , RATS , REGENERATION(PHYSIOLOGY) , REPRINTS , RESPONSE(BIOLOGY) , SURGERY


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE