Accession Number : ADA628095


Title :   Lecithin:retinol acyltransferase in ARPE-19


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Trevino, Simon G ; Schuschereba, Steven T ; Bowman, Phillip D ; Tsin, Andrew


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a628095.pdf


Report Date : 05 Apr 2005


Pagination or Media Count : 5


Abstract : The purpose of this study is to investigate if a readily available cell line (APRE 19) may be used to study in vitro function of visual cycle enzymes such as lecithin:retinol acyltransferase (LRAT). Cells incubated with exogenous retinol accumulated intracellular all trans retinol and all trans retinyl ester. Membrane proteins from ARPE 19 exhibited LRAT activity, which was inhibited by an LRAT inhibitor, retinyl bromoacetate (RBA). Gene microarray and Western blot results indicated that ARPE 19 cells expressed LRAT transcript and the LRAT protein. Therefore, our data show that ARPE 19 contains an active LRAT enzyme and suggest that it is an appropriate cell system to study visual cycle enzymes.


Descriptors :   *IN VITRO ANALYSIS , *LECITHIN , *VISION , ASSAYING , CELLS(BIOLOGY) , COUPLING(INTERACTION) , ENZYMES , EPITHELIUM , ESTERS , EYE PIGMENTS , GENES , INHIBITORS , MEMBRANES(BIOLOGY) , MORPHOLOGY , OPHTHALMOLOGY , PROTEINS , REGENERATION(PHYSIOLOGY) , RETINA , RIBONUCLEIC ACIDS , RODS , VITAMIN A


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE