Accession Number : ADA627756


Title :   Cytoprotection of Human Endothelial Cells From Menadione Cytotoxicity by Caffeic Acid Phenethyl Ester: The Role of Heme Oxygenase-1


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Wang, Xinyu ; Stavchansky, Salomon ; Zhao, Baiteng ; Bynum, James A ; Kerwin, Sean M ; Bowman, Phillip D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a627756.pdf


Report Date : 08 Jun 2008


Pagination or Media Count : 9


Abstract : Caffeic Acid Phenethyl Ester (CAPE), derived from various plant sources, has been shown to ameliorate ischemia/reperfusion injury in vivo , and this has been attributed to its ability to reduce oxidative stress. Here we investigated the cytoprotection of CAPE against menadione-induced oxidative stress in human umbilical vein endothelial cells (HUVEC) to evaluate potential gene expression involvement. CAPE exhibited dose- dependent cytoprotection of HUVEC. A gene screen with microarrays was performed to identify the potential cytoprotective gene(s) induced by CAPE. Heme oxygenase-1 (HO-1) was highly upregulated by CAPE and this was confirmed with reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting. Inhibition of HO-1 activity using the HO-1 inhibitor tin protoporphyrin IX (SnPPIX), resulted in loss of cytoprotection. Carbon monoxide, one of HO-1 catabolic products appeared to play a small role in CAPE protection. Caffeic acid, a potential metabolite of CAPE with similar free radical scavenging ability, however, didn't show any cytoprotective effect nor induce HO-1. These findings suggest an important role of HO-1 induction in CAPE cytoprotection against oxidant stress, which may not relate to CAPE structural antioxidant activity nor to its traditional enzymatic activity in decomposing heme but to a yet to be determined activity.


Descriptors :   *CELLS(BIOLOGY) , *CYTOTOXINS , *ENDOTHELIUM , ANTIOXIDANTS , ASSAYING , CATABOLISM , CULTURES(BIOLOGY) , CYTOLOGY , DOSAGE , ELECTROPHORESIS , ENZYMES , FREE RADICALS , GENE EXPRESSION , HEMOGLOBIN , IN VITRO ANALYSIS , IN VIVO ANALYSIS , ISCHEMIA , METABOLITES , MORBIDITY , ORGANS(ANATOMY) , OXIDATION , PATHOLOGY , PERFUSION , RIBONUCLEIC ACIDS , STATISTICAL ANALYSIS , TRAUMA , WOUNDS AND INJURIES


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research
      Toxicology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE