Accession Number : ADA627696


Title :   Functional Deficits and Insulin-Like Growth Factor-I Gene Expression Following Tourniquet-Induced Injury of Skeletal Muscle in Young and Old Rats


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Hammers, David W ; Merritt, Edward K ; Matheny, Wayne ; Adamo, Martin L ; Walters, Thomas J ; Estep, J S ; Farrar, Roger P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a627696.pdf


Report Date : 31 Jul 2008


Pagination or Media Count : 9


Abstract : This study investigated the effect of age on recovery of skeletal muscle from an ischemia-reperfusion (I/R)-induced injury. Young (6 mo old) and old (24 27 mo old) Sprague-Dawley rats underwent a 2-h bout of hindlimb ischemia induced by a pneumatic tourniquet (TK). The TK was released to allow reperfusion of the affected limb, and animals were divided into 7- and 14-day recovery groups. Maximum plantar flexor force production was assessed in both 7- and 14-day recovery groups of both ages, followed by histological evaluation. Subsequent analysis of IGF-I gene expression and intracellular signaling in 7-day recovery muscles was performed by RT-PCR and Western blotting, respectively. Old rats had significantly greater deficits in force production and exhibited more evidence of histological pathology than young at both 7 and 14 days postinjury. In addition, old rats demonstrated an attenuated upregulation of IGF-I mRNA and induction of proanabolic signaling compared with young in response to injury. We conclude that aged skeletal muscle exhibits more damage and/or defective regeneration following I/R and identify an age-associated decrease in local IGF-I responsiveness as a potential mechanism for this phenomenon.


Descriptors :   *ISCHEMIA , *MUSCLES , *RATS , *REGENERATION(ENGINEERING) , *WOUNDS AND INJURIES , AGING(PHYSIOLOGY) , CELLS(BIOLOGY) , CLINICAL MEDICINE , DAMAGE CONTROL , DRESSINGS , EXTREMITIES , GENE EXPRESSION , HEALING , HISTOLOGY , INSULIN , MEDICAL EQUIPMENT , MUSCULOSKELETAL SYSTEM , PATHOLOGY , PERFUSION , PHYSIOLOGICAL EFFECTS , RECOVERY , REGENERATION(PHYSIOLOGY) , RESPONSE(BIOLOGY) , RIBONUCLEIC ACIDS


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research
      Medical Facilities, Equipment and Supplies


Distribution Statement : APPROVED FOR PUBLIC RELEASE