Accession Number : ADA627691


Title :   Deactivating Chemical Agents Using Enzyme-Coated Nanofibers Formed by Electrospinning


Descriptive Note : Journal article


Corporate Author : ARMY NATICK SOLDIER RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA


Personal Author(s) : Han, Daewoo ; Kirby, Romy ; Filocamo, Shaun F ; Steckl, Andrew


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a627691.pdf


Report Date : Jan 2016


Pagination or Media Count : 38


Abstract : The coaxial electrospinning technique was investigated as a novel method to create stabilized, enzyme-containing fibers that have the potential to provide enhanced protection from chemical agents. Electrospinning is a versatile technique for the fabrication of polymer fibers with large length (cm to km): diameter (nm to um) aspect ratios. The large surface to volume ratios, along with the biofriendly nature of this technique, enables the fabrication of fiber mats with high enzyme concentrations, which amplify the catalytic activity per unit volume of membrane. Blended composite (single-source) fibers incorporate enzyme throughout the fiber, which may limit substrate accessibility to the enzyme. In contrast, core/sheath fibers can be produced by coaxial electrospinning with very high enzyme loading (80%) in the sheath without noticeable loss of enzymatic activity. Several core-sheath combinations have been explored with the toxin-mitigating enzyme DFPase in order to achieve fibers with optimum properties. The concentration of fluoride released, normalized for the amount of protein incorporated into the sheath, was used as a measure of the enzyme activity versus time. The coaxial core/sheath combination of PEO and DFPase produced the highest activity (7.3 mM/mg).


Descriptors :   *CHEMICAL AGENTS , *ENZYMES , *FIBERS , *NEUTRALIZATION , COATINGS , DECONTAMINATION , MATS , NANOSTRUCTURES , POLYMERS , SPINNING(INDUSTRIAL PROCESSES)


Subject Categories : Polymer Chemistry
      Textiles
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE