Accession Number : ADA626094


Title :   Radiolabeled Exosomes for the Early Detection of Metastases and to Predict Breast Cancer Premetastatic Niche


Descriptive Note : Annual rept. 1 Aug 2014-31 Jul 2015


Corporate Author : CORNELL UNIV MEDICAL COLL (WEILL) NEW YORK


Personal Author(s) : Vallabhajosula, Shankar ; Lyden, David ; Selgas, Hector P ; Nikolopoulou, Anastasia


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a626094.pdf


Report Date : 31 Aug 2015


Pagination or Media Count : 19


Abstract : Since current diagnostic tools fail to detect breast cancer (BC) metastatic spread at a very early stage, we proposed a new approach for the early detection of metastatic disease in BC patients using cancer cell derived particles known as exosomes as a guide. We hypothesized that exosomes tagged with appropriate radionuclides could be used as molecular imaging probes to study and non-invasively image metastatic spread, and to detect pre-metastatic niches using single-photon emission computed tomography (SPECT) or Positron emission tomography (PET). During this report period we have: 1) isolated and characterized exosomes from a series of BC cell lines of different metastatic capacity and tropism, 2) radiolabeled isolated BC exosomes with different radionuclides, such as 111In, 64Cu, 89Zr. We plan to 3) study the uptake and subsequent intracellular transport of radiolabeled exosomes by different cell types involved in pre-metastatic niche formation, 4) to study the in vivo stability, pharmacokinetics and biodistribution of radiolabeled exosomes following intravenous administration in naive and pathological mouse models. 5) Study the biodistribution and tumor uptake of radiolabeled exosomes (derived from peripheral blood of BC patients) in BC.


Descriptors :   *BIOLOGICAL DETECTION , *BREAST CANCER , *METASTASIS , BIOSTATISTICS , CELLS(BIOLOGY) , CLINICAL MEDICINE , COMPUTERIZED TOMOGRAPHY , DIAGNOSIS(MEDICINE) , DIAGNOSTIC EQUIPMENT , IN VIVO ANALYSIS , LABELED SUBSTANCES , MICE , NEOPLASMS , PATHOPHYSIOLOGY , PATIENTS , PHARMACOKINETICS , POSITRON EMISSION TOMOGRAPHY , PROTEINS


Subject Categories : Biochemistry
      Medicine and Medical Research
      Medical Facilities, Equipment and Supplies


Distribution Statement : APPROVED FOR PUBLIC RELEASE