Accession Number : ADA623593


Title :   EphB1 as a Novel Drug Target to Combat Pain and Addiction


Descriptive Note : Annual rept. 1 Sep 2014-31 Aug 2015


Corporate Author : TEXAS UNIV AT DALLAS SOUTHWESTERN MEDICAL CENTER


Personal Author(s) : Henkemeyer, Mark


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a623593.pdf


Report Date : Sep 2015


Pagination or Media Count : 11


Abstract : We have shown using knockout mice and other methods that the neuronal/synaptic receptor protein known as EphB1 is a central player in nerve injury-induced chronic neuropathic pain as well as the related pain symptoms associated with the withdrawal from opioid/morphine addiction. Our hypothesis is that EphB1 participates in pain caused by nerve damage and opioid withdrawal through the ability of its extracellular domain to form protein-protein interactions with the NR1 subunit of the NMDA receptor and inappropriately strengthen the synapses and neural circuits in the spinal cord that transmit pain signals up into the brain. Our project is to carry out high-throughput screens (HTS) to identify small molecular weight drug-like compounds that antagonize the EphB1:NR1 protein-protein interaction. In year 1 of the project we have cloned, expressed, and purified the unique protein tools needed for the project. We have also worked to develop protein-protein interaction assays necessary for the HTS.


Descriptors :   *PROTEINS , DRUGS , NERVES , PAIN , RECEPTOR SITES(PHYSIOLOGY)


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE