Accession Number : ADA623185


Title :   Renewable Bio-Solar Hydrogen Production: The Second Generation (Part B)


Descriptive Note : Final rept. 15 May 2011-31 Dec 2014


Corporate Author : PENNSYLVANIA STATE UNIV STATE COLLEGE DEPT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY


Personal Author(s) : Bryant, Donald A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a623185.pdf


Report Date : 20 Mar 2015


Pagination or Media Count : 13


Abstract : In this project we used targeted mutagenesis, overexpression of genes, transcription profiling, and metabolic profiling of the cyanobacterium Synechococcus sp. PCC 7002 to understand better how to optimize the production of biohydrogen and potential carbon-based biofuel molecules. A major finding was that the tricarboxylic acid (TCA) cycle is not branched, as had been believed for nearly 50 years, but is in fact closed by two noncanonical enzymes that replace 2-oxoglutarate dehydrogenase. In addition to redefining the TCA cycle, we validated the occurrence of the glyoxylate cycle and the gamma-aminobutyric acid (GABA) shunt in a few cyanobacteria, and we studied the partitioning of metabolites between the glycolytic and oxidative pentose phosphate pathways. Important transcription regulators, including RbcR, Fur, and ChlR, were identified and characterized. Approaches to maximize biohydrogen production and carbon-based biofuels (e.g., cellulose, poly-hydroxybutyrate) were characterized and optimized. These studies yielded important new biochemical details about critically important aspects of cyanobacterial physiology and metabolism.


Descriptors :   *BACTERIA , BIOMASS , CELLULOSE , FERMENTATION , GENE EXPRESSION , METABOLISM , PHOTOSYNTHESIS


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE