Accession Number : ADA622570


Title :   In Vitro Studies of Primary Explosive Blast Loading on Neurons


Descriptive Note : Journal article


Corporate Author : ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD WEAPONS AND MATERIALS RESEARCH DIRECTORATE


Personal Author(s) : Zander, Nicole E ; Piehler, Thuvan ; Boggs, Mary E ; Banton, Rohan ; Benjamin, Richard


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a622570.pdf


Report Date : Sep 2015


Pagination or Media Count : 16


Abstract : In a military setting, traumatic brain injury (TBI) is frequently caused by blast waves that can trigger a series of neuronal biochemical changes. Although many animal models have been used to study the effects of primary blast waves, elucidating the mechanisms of damage in a whole-animal model is extremely complex. In vitro models of primary blast, which allow for the deconvolution of mechanisms, are relatively scarce. It is largely unknown how structural damage at the cellular level impacts the functional activity at variable time scales after the TBI event. A novel in vitro system was developed to probe the effects of explosive blast (ranging from 25 to 40 psi) on dissociated neurons. PC12 neurons were cultured on laminin-coated substrates, submerged underwater, and subjected to single and multiple blasts in a controlled environment. Changes in cell membrane permeability, viability, and cell morphology were evaluated. Significant increases in axonal beading were observed in the injured cells. In addition, although cell death was minimal after a single insult, cell viability decreased significantly following repeated blast exposure.


Descriptors :   *BLAST LOADS , *NERVE CELLS , *TRAUMATIC BRAIN INJURIES , BLAST , BLAST WAVES , CELLS(BIOLOGY) , EXPLOSIVES , EXPOSURE(PHYSIOLOGY) , IN VITRO ANALYSIS


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Explosions


Distribution Statement : APPROVED FOR PUBLIC RELEASE