Accession Number : ADA622412


Title :   Genome Annotation and Transcriptomics of Oil-Producing Algae


Descriptive Note : Final rept. 1 Apr 2010-31 Dec 2014


Corporate Author : CALIFORNIA UNIV LOS ANGELES


Personal Author(s) : Merchant, Sabeeha ; Pellegrini, Matteo


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a622412.pdf


Report Date : 16 Mar 2015


Pagination or Media Count : 10


Abstract : Most algae accumulate triacylglycerols (TAGs) when they are starved for essential nutrients like N, S, P (or Si in the case of some diatoms). We had proposed to use whole transcriptome analyses to detail the changes in gene expression that occur during N-starvation induced TAG accumulation in Chlamydomonas. We used RNA-Seq on the Illumina platform for quantitative determination of the Chlamydomonas transcriptome. Deep coverage over multiple time points in nearly a dozen different experiments in at least 3 strains allowed us to distinguish early responses to the stress signal and to unequivocally identify relevant changes in RNA abundance. This led to the functional identification of 8 acyltransferases, several of which were documented to have a role in TAG accumulation in stressed algal cells, and candidate regulators of the N starvation response. We used our RNA-Seq data analysis pipeline for building and quantitating transcripts from Chlorella and Cyclotella in TAG-producing conditions and we assembled a draft genome for Cyclotella. During a period of project extension, we generated new strand-specific RNA-Seq data from ribosomal RNA-depleted preparations of RNA for the purpose of identifying regulatory lncRNAs.


Descriptors :   *ALGAE , *GENE EXPRESSION , *STARVATION , DIESEL FUELS , GENOME , NUTRIENTS , OILS , RIBONUCLEIC ACIDS , TRANSCRIPTION(GENETICS)


Subject Categories : Genetic Engineering and Molecular Biology
      Biology
      Stress Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE