Accession Number : ADA620823


Title :   Antibiofilm and Antimicrobial Efficacy of DispersinB (registered trademark)-KSL-W Peptide-Based Wound Gel Against Chronic Wound Infection Associated Bacteria


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Gawande, Purushottam V ; Leung, Kai P ; Madhyastha, Srinivasa


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a620823.pdf


Report Date : 21 Jan 2014


Pagination or Media Count : 9


Abstract : The medical importance of bacterial biofilms has increased with the recognition of biofilms as one of the major contributors to the slow or non-healing chronic wounds such as diabetic foot ulcers, venous leg ulcers, and pressure ulcers. Being a protected community of microorganisms, biofilms are notoriously refractory to antibiotic treatments. As the conventional treatment modalities have proven ineffective, this study provides the in vitro evidence to support the use of a novel combination of DispersinB antibiofilm enzyme that inhibits biofilm formation and disperses preformed biofilm, and thus making the biofilm bacteria more susceptible to a broad-spectrum KSL-W antimicrobial peptide. The combination of Silver-Sept(TM), DispersinB(registered trademark) and KSL-W(registered trademark) peptide showed synergistic antibiofilm and antimicrobial activity against chronic wound infection associated biofilm-embedded bacteria such as Methicillin- resistant Staphylococcus aureus (MRSA) ,Staphylococcus epidermidis, Coagulase-negative Staphylococci (CoNS), and Acinetobacter baumannii. In addition, the wound gel formulation comprising DispersinB, KSL-W peptide based-wound gel was significantly more effective in inhibiting the bio- film-embedded MRSA , S. epidermidis, CoNS, Vancomycin-resistant Enterococci, A. baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa (P less than 0.05). Thus, this study provides promising evidence for the potential application of antibiofilm-antimicrobial DispersinB(registered trademark) -KSL-W wound gel in chronic wound management.


Descriptors :   *ANTIMICROBIAL AGENTS , *GELS , *PEPTIDES , *WOUNDS AND INJURIES , ASSAYING , BACTERIA , CLINICAL MEDICINE , CULTURES(BIOLOGY) , DIAGNOSIS(MEDICINE) , DRESSINGS , ENZYMES , EXPOSURE(PHYSIOLOGY) , HEALING , IN VITRO ANALYSIS , INFECTIOUS DISEASES , KLEBSIELLA PNEUMONIAE , MICROORGANISMS , ORGANIC MATERIALS , PSEUDOMONAS AERUGINOSA , QUANTITATIVE ANALYSIS , REGENERATION(PHYSIOLOGY) , STAPHYLOCOCCUS AUREUS , TRAUMA , ULCERS , VEINS


Subject Categories : Biochemistry
      Medicine and Medical Research
      Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE