Accession Number : ADA620815


Title :   Effect of Cell-seeded Hydroxyapatite Scaffolds on Rabbit Radius Bone Regeneration


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Rathbone, C R ; Guda, T ; Singleton, B M ; Oh, D S ; Appleford, M R ; Ong, J L ; Wenke, J C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a620815.pdf


Report Date : 22 Jun 2013


Pagination or Media Count : 11


Abstract : Highly porous hydroxyapatite (HA) scaffolds were developed as bone graft substitutes using a template coating process, characterized, and seeded with bone marrow- derived mesenchymal stem cells (BMSCs). To test the hypothesis that cell-seeded HA scaffolds improve bone regeneration, HA scaffolds without cell seeding (HA-empty), HA scaffolds with 1.5 X 10(exp4) BMSCs (HA-low), and HA scaffolds with 1.5 X 10(exp6) BMSCs (HA-high) were implanted in a 10-mm rabbit radius segmental defect model for 4 and 8 weeks. Three different fluorochromes were administered at 2, 4, and 6 weeks after implantation to identify differences in temporal bone growth patterns. It was observed from fluorescence histomorphometry analyses that an increased rate of bone infiltration occurred from 0 to 2 weeks (p less than 0.05) of implantation for the HA-high group (2.9 + or - 0.5 mm) as compared with HA-empty (1.8 + or - 0.8 mm) and HA-low (1.3 + or - 0.2 mm) groups. No significant differences in bone formation within the scaffold or callus formation was observed between all groups after 4 weeks, with a significant increase in bone regenerated for all groups from 4 to 8 weeks (28.4% across groups). Although there was no difference in bone formation within scaffolds, callus formation was significantly higher in HA-empty scaffolds (100.9 + or - 14.1 mm(exp3)) when compared with HA-low (57.8 6 7.3 mm(exp 3) ; p less than or equal to 0.003) and HA-high (69.2 + or 110.4 mm 3 ; p less than or equal to 0.02) after 8 weeks. These data highlight the need for a better understanding of the parameters critical to the success of cell-seeded HA scaffolds for bone regeneration.


Descriptors :   *BONE MARROW , *REGENERATION(PHYSIOLOGY) , BIOMECHANICS , CONNECTIVE TISSUE , CULTURES(BIOLOGY) , EMBRYOS , EXPERIMENTAL DESIGN , FLUORESCENCE , GROWTH(PHYSIOLOGY) , HISTOLOGY , IMPLANTATION , INFILTRATION(FLUIDS) , MORPHOLOGY(BIOLOGY) , POROUS MATERIALS , RABBITS , SEEDING , SKIN GRAFTS , STATISTICAL ANALYSIS , STEM CELLS , TEST AND EVALUATION , TISSUES(BIOLOGY) , TRANSPLANTATION


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Biomedical Instrumentation and Bioengineering


Distribution Statement : APPROVED FOR PUBLIC RELEASE