Accession Number : ADA620515


Title :   A Transient Cell-shielding Method for Viable MSC Delivery Within Hydrophobic Scaffolds Polymerized in situ


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Guo, Ruijing ; Ward, Catherine L ; Davidson, Jeffrey M ; Duvall, Craig L ; Wenke, Joseph C ; Guelcher, Scott A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a620515.pdf


Report Date : 27 Mar 2015


Pagination or Media Count : 14


Abstract : Cell-based therapies have emerged as promising approaches for regenerative medicine. Hydrophobic poly(ester urethane)s offer the advantages of robust mechanical properties, cell attachment without the use of peptides, and controlled degradation by oxidative and hydrolytic mechanisms. However, the application of injectable hydrophobic polymers to cell delivery is limited by the challenges of protecting cells from reaction products and creating a macroporous architecture post-cure. We designed injectable carriers for cell delivery derived from reactive, hydrophobic polyisocyanate and polyester triol pre-cursors. To overcome cell death caused by reaction products from in situ polymerization, we encapsulated bone marrow-derived stem cells (BMSCs) in fastdegrading, oxidized alginate beads prior to mixing with the hydrophobic precursors. Cells survived the polymerization at greater than 70% viability, and rapid dissolution of oxidized alginate beads after the scaffold cured created interconnected macropores that facilitated cellular adhesion to the scaffold in vitro. Applying this injectable system to deliver BMSCs to rat excisional skin wounds showed that the scaffolds supported survival of transplanted cells and infiltration of host cells, which improved new tissue formation compared to both implanted, pre-formed scaffolds seeded with cells and acellular controls. Our design is the first to enable injectable delivery of settable, hydrophobic scaffolds where cell encapsulation provides a mechanism for both temporary cytoprotection during polymerization and rapid formation of macropores post-polymerization. This simple approach provides potential advantages for cell delivery relative to hydrogel technologies, which have weaker mechanical properties and require incorporation of peptides to achieve cell adhesion and degradability.


Descriptors :   *IN VIVO ANALYSIS , *POLYMERIZATION , *STEM CELLS , BIOCHEMISTRY , BIOMATERIALS , BIOMOLECULES , BONE MARROW , COMPUTERIZED SIMULATION , CULTURES(BIOLOGY) , HEALING , HOSTS(BIOLOGY) , HYDROLYSIS , HYDROPHOBIC PROPERTIES , OXIDATION , PEPTIDES , PERMEABILITY , POLYURETHANE RESINS , REACTANTS(CHEMISTRY) , REGENERATION(PHYSIOLOGY) , SURVIVAL(GENERAL) , SYNTHESIS(CHEMISTRY) , THERAPY , TISSUES(BIOLOGY) , URETHANES , VIABILITY , WOUNDS AND INJURIES


Subject Categories : Biochemistry
      Medicine and Medical Research
      Biomedical Instrumentation and Bioengineering


Distribution Statement : APPROVED FOR PUBLIC RELEASE