Accession Number : ADA619691


Title :   The Isolation and Characterization of Human Prostate Cancer Stem Cells


Descriptive Note : Final rept. 1 Aug 2008-28 Feb 2015


Corporate Author : MICHIGAN UNIV ANN ARBOR SCHOOL OF MEDICINE


Personal Author(s) : Palapattu, Ganesh S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a619691.pdf


Report Date : May 2015


Pagination or Media Count : 84


Abstract : The overall objective of this proposal is to develop a durable cure for lethal prostate cancer through the elucidation of the role of cancer stem cells in the pathogenesis of the disease. During the past year, we have made the following significant findings/observations: i) 3D culture of human prostate cancer cells with magnetic nanoparticles is not optimal for tumor initiation studies, ii) in vitro co-culture of human prostate cancer cells (established cell lines and primary patient samples) with human prostate fibroblasts hold promise as models of tumor initiation/cancer stem cell activity. We continue to optimize and validate our in vitro model of prostate cancer initiation to facilitate cancer stem cell discovery as well as drug targeting.


Descriptors :   *PROSTATE CANCER , *STEM CELLS , ANDROGENS , BIOASSAY , CLINICAL MEDICINE , CULTURES(BIOLOGY) , DRUG TOLERANCE , ENDOCRINE GLANDS , FIBROBLASTS , GENE EXPRESSION , GENETIC MARKERS , GROWTH(PHYSIOLOGY) , IMMUNOTHERAPY , IN VITRO ANALYSIS , INCUBATION , LETHALITY , MICROBIOLOGY , NANOPARTICLES , NEOPLASMS , PATHOGENESIS , RECEPTOR SITES(PHYSIOLOGY) , TISSUES(BIOLOGY)


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE