Accession Number : ADA619575


Title :   Identification and Transcription Profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): Developmental Regulation and Environmental Response


Descriptive Note : Journal article


Corporate Author : AGRICULTURAL RESEARCH SERVICE GAINESVILLE FL CENTER FOR MEDICAL AGRICULTURAL AND VETERINARY ENTOMOLOGY


Personal Author(s) : Zhao, Liming ; Kline, Daniel L ; Becnel, James J ; Chen, Jian ; Allan, Sandra A ; Clark, Gary G ; Linthicum, Kenneth J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a619575.pdf


Report Date : 18 Dec 2014


Pagination or Media Count : 13


Abstract : The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus) taeniorhynchus Wiedemann has been cloned and sequenced. The 824 bp full-length mRNA sequence of AetNDUFS8 encodes an open reading region of 651 bp (that is, 217 amino acids). To identify if AetNDUFS8 is developmentally regulated, we employed a quantitative real-time polymerase chain reaction to examine AetNDUFS8 gene expression levels in all developmental stages of Ae. taeniorhynchus. In egg, larval, and pupal stages, AetNDUFS8 was expressed at relatively low levels. However, in the teneral adult stage, AetNDUFS8 was highly expressed. During the time course study in response to permethrin pesticide treatment, quantitative real-time polymerase chain reaction (PCR) also showed that mRNA transcription levels of AetNDUFS8 were regulated in female Ae. taeniorhynchus. A mitochondrially encoded NADH dehydrogenase subunit 5 AetNADH5 was highly expressed in different developmental stages of Ae. taeniorhynchus. This study suggests that AetNDUFS8 and AetNADH5 play an essential role in the development of Ae. taeniorhynchus and will provide information useful for developing dsRNA pesticide for mosquito control.


Descriptors :   *AEDES , *INSECTICIDES , *TRANSCRIPTION(GENETICS) , GENE EXPRESSION , IDENTIFICATION , RIBONUCLEIC ACIDS


Subject Categories : Agricultural Chemistry
      Biochemistry
      Genetic Engineering and Molecular Biology
      Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE