Accession Number : ADA619194


Title :   Impairment of IGF-I Expression and Anabolic Signaling Following Ischemia/Reperfusion in Skeletal Muscle of Old Mice


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Hammers, David W ; Matheny Jr, Ronald W ; Sell, Christian ; Adamo, Martin L ; Walters, Thomas J ; Estep, J S ; Farrar, Roger P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a619194.pdf


Report Date : 01 Apr 2011


Pagination or Media Count : 21


Abstract : With the advancement of age, skeletal muscle undergoes a progressive decline in mass, function, and regenerative capacity. Previously, our laboratory has reported an age-reduction in recovery and local induction of IGF-I gene expression with age following tourniquet (TK)-induced skeletal muscle ischemia/reperfusion (I/R). In this study, young (6 mo) and old (24 28 mo) mice were subjected to 2 hours of TK-induced ischemia of the hindlimb followed by 1, 3, 5, or 7 days of reperfusion. Real time-PCR analysis revealed clear age-related reductions and temporal alterations in the expression of IGF-I and individual IGF-I Ea and Eb splice variants. ELISA verified a reduction of IGF-I peptide with age following 7 days recovery from TK. Western blotting showed that the phosphorylation of Akt, mTOR, and FoxO3, all indicators of anabolic activity, were reduced in the muscles of old mice. These data indicate an age-related impairment of IGF-I expression and intracellular signaling does exist following injury, and potentially has a role in the impaired recovery of skeletal muscle with age.


Descriptors :   *AGING(PHYSIOLOGY) , *MUSCLES , *REGENERATION(PHYSIOLOGY) , GENE EXPRESSION , ISCHEMIA , MICE , PEPTIDES , PERFUSION


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE