Accession Number : ADA617428


Title :   Swarming Mechanisms in the Yellow Fever Mosquito: Aggregation Pheromones are Involved in the Mating Behavior of Aedes aegypti


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH UNIT NO 3 FPO NEW YORK 09527


Personal Author(s) : Fawaz, Emadeldin Y ; Allan, Sandra A ; Bernier, Ulrich R ; Obenauer, Peter J ; Diclaro II, Joseph W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a617428.pdf


Report Date : Dec 2014


Pagination or Media Count : 9


Abstract : Mosquitoes of various species mate in swarms comprised of tens of thousands of flying males. In this study, we examined Aedes aegypti swarming behavior and identified associated chemical cues. Novel evidence is provided that Ae. aegypti females aggregate by means of olfactory cues, such as aggregation pheromones. Isolation of Ae. aegypti aggregation pheromones was achieved by aeration of confined mosquitoes and collection of associated volatiles by glass filters. The collected volatiles were identified through gas chromatography mass spectrometry (GCMS). Three aggregation pheromones were collected and identified as 2,6,6-trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) (CAS# 1125-21-9, tR = 18.75), 2,2,6-trimethylcyclohexane-1,4- dione (the saturated analog of ketoisophorone) (CAS# 20547-99-3, tR = 20.05), and 1-(4-ethylphenyl) ethanone (CAS# 937-30- 4, tR = 24.22). Our biological studies revealed that the identified compounds stimulated mosquito behavior under laboratory conditions. The mechanism of mosquito swarm formation is discussed in light of our behavioral study findings. A preliminary field trial demonstrated the potential application of the isolated aggregation pheromones in controlling Ae. aegypti.


Descriptors :   *CULICIDAE , *PHEROMONES , *REPRODUCTION(PHYSIOLOGY) , BIOASSAY , FIELD TESTS , OLFACTOMETERS , STATISTICAL ANALYSIS , YELLOW FEVER


Subject Categories : Biology
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE