Accession Number : ADA615764


Title :   The Role of Endothelial Cells in Myofiber Differentiation and the Vascularization and Innervation of Bioengineered Muscle Tissue in vivo


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Criswell, Tracy L ; Corona, Benjamin T ; Wang, Zhan ; Zhou, Yu ; Niu, Guoguang ; Xu, Yong ; Christ, George J ; Soker, Shay


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a615764.pdf


Report Date : 08 Oct 2012


Pagination or Media Count : 11


Abstract : Musculoskeletal disorders are a major cause of disability and effective treatments are currently lacking. Tissue engineering affords the possibility of new therapies utilizing cells and biomaterials for the recovery of muscle volume and function. A major consideration in skeletal muscle engineering is the integration of a functional vasculature within the regenerating tissue. In this study we employed fluorescent cell labels to track the location and differentiation of co-cultured cells in vivo and in vitro. We first utilized a co-culture of fluorescently labeled endothelial cells (ECs) and muscle progenitor cells (MPCs) to investigate the ability of ECs to enhance muscle tissue formation and vascularization in an in vivo model of bioengineered muscle. Scaffolds that had been seeded with both MPCs and ECs showed significantly greater vascularization, tissue formation and enhanced innervation as compared to scaffolds seeded with MPCs alone. Subsequently, we performed in vitro experiments using a 3-cell type system (ECs, MPCs, and pericytes (PCs)) to demonstrate the utility of fluorescent cell labeling for monitoring cell growth and differentiation. The growth and differentiation of individual cell types was determined using live cell fluorescent microscopy demonstrating the utility of fluorescent labels to monitor tissue organization in real time.


Descriptors :   *BIOENGINEERING , *ENDOTHELIUM , *MUSCULOSKELETAL DISEASES , ANGIOGENESIS , BIOMATERIALS , CELLS(BIOLOGY) , CULTURES(BIOLOGY) , FLUORESCENT ANTIBODY TECHNIQUES , HISTOLOGY , IMMUNOCHEMISTRY , IN VITRO ANALYSIS , IN VIVO ANALYSIS , MICROSCOPY , MUSCLES , REGENERATION(PHYSIOLOGY) , TISSUES(BIOLOGY)


Subject Categories : Medicine and Medical Research
      Biomedical Instrumentation and Bioengineering


Distribution Statement : APPROVED FOR PUBLIC RELEASE