Accession Number : ADA614937


Title :   The Breast Cancer DNA Interactome


Descriptive Note : Final rept. 30 Sep 2011-29 Sep 2014


Corporate Author : PALO ALTO INSTITUTE FOR RESEARCH AND EDUCATION INC CA


Personal Author(s) : Hoffman, Andrew R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a614937.pdf


Report Date : Dec 2014


Pagination or Media Count : 39


Abstract : Gene transcription may be regulated by remote enhancer regions through chromosome looping. The role that higher order chromatin organization plays in cancer progression and metastasis is not yet fully understood. Our major goal has been to characterize physical interactions among selected breast cancer gene loci in normal and malignant mammary cell lines. We used IGFBP3, a gene that has been involved in breast cancer pathogenesis, as bait in a 4C-seq experiment comparing normal breast cells (HMEC) and two different breast cancer cell lines (MCF7, an ER positive cell line and MDA-MB-231, a triple negative cell line). We found that in HMEC the genes BCAS1-4 (breast carcinoma amplified sequence 1-4) were found in the top 10 most significantly enriched regions for interactions with IGFBP3. We also found EGFR (epidermal growth factor receptor), a gene that has also been implicated in cancer, to interact significantly with IGFBP3 in all three samples. We also characterized IRAIN, a noncoding RNA important in long range interactions. These data suggest an important role for chromosomal interactions in the pathogenesis of breast cancer.


Descriptors :   *BREAST CANCER , *CELLS(BIOLOGY) , *CHROMOSOMES , *DEOXYRIBONUCLEIC ACIDS , *GENES , *PATHOGENESIS , CHROMATIN , INTERACTIONS , METASTASIS , TRANSCRIPTION(GENETICS)


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE