Accession Number : ADA614664


Title :   C. albicans Growth, Transition, Biofilm Formation, and Gene Expression Modulation by Antimicrobial Decapeptide KSL-W


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX ARMY DENTAL AND TRAUMA RESEARCH DETACHMENT


Personal Author(s) : Theberge, Simon ; Semlali, Abdelhabib ; Alamri, Abdullah ; Leung, Kai P ; Rouabhia, Mahmoud


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a614664.pdf


Report Date : 07 Nov 2013


Pagination or Media Count : 15


Abstract : Background: Antimicrobial peptides have been the focus of much research over the last decade because of their effectiveness and broad-spectrum activity against microbial pathogens. These peptides also participate in inflammation and the innate host defense system by modulating the immune function that promotes immune cell adhesion and migration as well as the respiratory burst, which makes them even more attractive as therapeutic agents. This has led to the synthesis of various antimicrobial peptides, including KSL-W (KKVVFWVKFK-NH2), for potential clinical use. Because this peptide displays antimicrobial activity against bacteria, we sought to determine its antifungal effect on C. albicans. Growth, hyphal form, biofilm formation, and degradation were thus examined along with EFG1, NRG1, EAP1, HWP1, and SAP 2-4-5-6 gene expression by quantitative RT-PCR. Results: This study demonstrates that KSL-W markedly reduced C. albicans growth at both early and late incubation times. The significant effect of KSL-W on C. albicans growth was observed beginning at 10 g/ml after 5 h of contact by reducing C. albicans transition and at 25 g/ml by completely inhibiting C. albicans transition. Cultured C. albicans under biofilm-inducing conditions revealed that both KSL-W and amphotericin B significantly decreased biofilm formation at 2, 4, and 6 days of culture. KSL-W also disrupted mature C. albicans biofilms. The effect of KSL-W on C. albicans growth, transition, and biofilm formation/disruption may thus occur through gene modulation, as the expression of various genes involved in C. albicans growth, transition and biofilm formation were all downregulated when C. albicans was treated with KSL-W. The effect was greater when C. albicans was cultured under hyphae-inducing conditions. Conclusions: These data provide new insight into the efficacy of KSL-W against C. albicans and its potential use as an antifungal therapy.


Descriptors :   *ANTIMICROBIAL AGENTS , *CANDIDA , *GENE EXPRESSION , *PEPTIDES , FILMS , GENES , GROWTH(PHYSIOLOGY) , PEPTIDE HYDROLASES , TRANSCRIPTION(GENETICS)


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Biology
      Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE